| Bioactivity | Trioxsalen (Trisoralen), a psoralen derivative, is a photochemical DNA crosslinker. Trioxsalen only works after photoactivation with near ultraviolet light. Trioxsalen is a photosensitizer that can be used for the research of vitiligo and hand eczema. Trioxsalen is used for visualization of genomic interstrand cross-links localized by laser photoactivation[1][2][3]. | ||||||||||||
| Invitro | Trioxsalen (200 nM) plus 365 nm light produce DNA crosslinks but not breaks in mouse L1210 leukemia cells[4].Trioxsalen/UVA treatment of HaCaT keratinocytes induces NF-kappaB binding activity, and that this is a synergistic effect[5].Trioxsalen shows anti-inflammatory capacities in vitro[5]. | ||||||||||||
| In Vivo | Trioxsalen induces dose-dependent depletion of ATPase-positive LCs, with a maximal depletion of 80%, in oral mucous membrane (OMM) with PUVA (UVA doses of 1-8 J/cm2) in rat[3]. | ||||||||||||
| Name | Trioxsalen | ||||||||||||
| CAS | 3902-71-4 | ||||||||||||
| Formula | C14H12O3 | ||||||||||||
| Molar Mass | 228.24 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. M T Chuan, et al. Effectiveness of psoralen photochemotherapy for vitiligo. J Formos Med Assoc. 1999 May;98(5):335-40. [2]. A Marco van Coevorden, et al. Comparison of oral psoralen-UV-A with a portable tanning unit at home vs hospital-administered bath psoralen-UV-A in patients with chronic hand eczema: an open-label randomized controlled trial of efficacy. Arch Dermatol. 200 [3]. A Kuusilehto, et al. The effect of PUVA on langerhans cells in rat oral epithelium photosensitized with systemic methoxsalen or topical trioxsalen. Photodermatol Photoimmunol Photomed. 2000 Jun;16(3):129-33. [4]. L F Cohen, et al. Interstrand DNA crosslinking by 4,5'8-trimethylpsoralen plus monochromatic ultraviolet light. Studies by alkaline elution in mouse L1210 leukemia cells. Biochim Biophys Acta. 1980 Nov 14;610(1):56-63. [5]. H Sánchez Ruderisch, et al. Trioxsalen in the presence of UVA is able to induce nuclear factor kappa B binding activity in HaCaT keratinocytes. Skin Pharmacol Appl Skin Physiol. Sep-Oct 2002;15(5):335-41. |