Sulforhodamine G_product_DataBase

Bioactivity	Sulforhodamine G is a fluorescent stain with broad dynamic ranges. Sulforhodamine G can be used for the research of protein stains[1].
Invitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling of fluorescent internal protein:A. Prepared of protein samples: 1. Protein samples to be analyzed are spiked with 0.1% of the total protein load of ALIS647 (ALIS internal standard) prior.2. Protein sample is separated with 2-DE in the dark.B. Purify Sulforhodamine G:1. Sulforhodamine G (60% purity, 10 mg) dissolved in 100 mL of 1% v/v acetic acid to purify by RP chromatography.2. Collect the pool with an absorbance maximum at 528 nm, lyophilized to dryness and stored as a dry powder at 47℃.C. Stainning:1. staining was performed in polypropylene staining dishes wrapped in aluminum foil to prevent photobleaching of the stains.2. Sulforhodamine G staining is performed overnight in 35% methanol with a four-fold molar excess of dye: protein based on an average protein molecular weight of 50 kDa.3. Following 4×15 min washes in 35% methanol and 2×15 min equilibrations in water.4. Total protein and ALIS are visualized with a laser scanner using different channels.5. Protein spots visualized using total protein stain (λex=532 nm) and ALIS (λex=633 nm) are quantified separately using 2-DE software, and statistical analysis.
Name	Sulforhodamine G
CAS	5873-16-5
Formula	C25H25N2Na2O7S2+
Molar Mass	575.58
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Asa M Wheelock, et al. Use of a fluorescent internal protein standard to achieve quantitative two-dimensional gel electrophoresis. Proteomics. 2006 Mar;6(5):1385-98.

PeptideDB

Sulforhodamine G

CAS: 5873-16-5 F: C25H25N2Na2O7S2+ W: 575.58