| Bioactivity | Propiolactone (β-propiolactone; 2-Oxetanone) is a viral chemical inactivator that causes the infectious inactivation of viruses. Propiolactone was co-incubated with SARS-CoV at a ratio of 1:1000 (v:v) and used as a bacteriostatic agent to formulate the BPL-inactivated influenza virus vaccine (Flu-BPL)[1][2]. | |||||||||
| Target | SARS-CoV-2 | |||||||||
| Invitro | Propiolactone (β-propiolactone) 可用于疫苗纯化。低速离心收获细胞后，用 Propiolactone (1:1000 v:v) 化学灭活 SARS-CoV。Propiolactone 与 SARS-CoV 在 4℃ 下孵育 24 小时。再在室温下进行第二次孵育，以水解残留的 Propiolactone。and concentration of the vaccine. Following BPL inactivation. a polvethylene glycol-sodium chloride (PEG-NaCl) mixture was added to precipitate the inactivated virus. After cen以及疫苗的浓度。在Propiolactone 失活之后，加入聚乙二醇-氯化钠 (PEG-NaCl) 混合物沉淀灭活病毒。最后加入 Propiolactone (1:10000 v:v) 作为抑菌剂。Propiolactone 灭活的病毒在 Vero 细胞中失去传染性[1]。 0 --> Propiolactone 相关抗体: | |||||||||
| In Vivo | 用 Propiolactone (β-propiolactone) 灭活的甲型流感病毒免疫小鼠 (~25 mg total protein per dose；肌肉注射)。Propiolactone 灭活处理后，SARS 对年轻 BALB/c 小鼠不致死。尽管病毒在小鼠的呼吸道中复制，但在第 5 天被清除。Propiolactone 处理得到 1.5 μg 总血凝素蛋白，使小鼠受感染后均为阴性[1]。 | |||||||||
| Name | Propiolactone | |||||||||
| CAS | 57-57-8 | |||||||||
| Formula | C3H4O2 | |||||||||
| Molar Mass | 72.06 | |||||||||
| Appearance | 液体 | |||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | |||||||||
| Storage |
|
|||||||||
| Reference | [1]. Roberts A, et al. Immunogenicity and protective efficacy in mice and hamsters of a β-propiolactone inactivated whole virus SARS-CoV vaccine. Viral Immunol. 2010 Oct;23(5):509-19 [2]. Kulkarni R, et al. Anti-SARS-CoV-2 IgG antibody response among Indian COVID-19 patients using β-propiolactone-inactivated, whole virus-based indirect ELISA. J Virol Methods. 2021 Jan;287:113996. |