LDS-751_product_DataBase

Bioactivity	Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 has a high affinity for DNA and fluorescence is enhanced after binding, but the maximum emission wavelength is 670nm. Lds-751 and Thiazole orange can be used for the differentiation of red blood cells, platelets, reticulocytes, and nucleated cells and can be stimulated at 488nm. Studies have shown that LDS-751 binds almost exclusively to mitochondria when incubated with nucleated living cells. After nucleated Acridine Orange (HY-101879) staining and LDS-751 treatment of cells, confocal microscopy revealed almost no co-location of the cells. Staining with Rhodamine 123 (HY-D0816), a dye known to bind polarized mitochondria, was almost identical to the pattern observed with LDS-751[1][2][3]. Storage: Keep away from light.
Invitro	使用方法工作液的配制1.1 制备 LDS-751 储存液用 ddH2O 配制 2 mg/mL 的 LDS-751。注：LDS-751 储存液建议分装后于 4℃ 或 -20℃ 避光保存。1.2 制备 LDS-751 工作液用预热好的无血清细胞培养基或 PBS 稀释储存液，按照 2 μg/mL 配置 LDS-751 工作液。注：请根据实际情况调整 LDS-751 工作液浓度，且现用现配。细胞染色2.1 细胞处理悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入 PBS 洗涤两次，每次 5 分钟。2.2 用 3.7% 甲醛固定细胞 10 分钟，吸去固定剂，用 PBS 冲洗三次，每次 5 分钟。2.3 加入 0.2% Triton X-100, 细胞透化处理 5 分钟，吸去 Triton X，PBS 清洗三次，每次 5 分钟。2.4 加入 1 mL LDS-751 工作液，室温避光孵育 1-5 分钟。2.5 400 g，4℃ 离心 3-4 分钟，弃去上清。2.6 加入 PBS 洗涤细胞两次，每次 5 分钟。2.7 用 1 mL 无血清培养基或 PBS 重悬细胞后，在荧光显微镜或流式细胞仪下检测。保存条件4℃ 避光保存一年。注意事项1. LDS-751 储存液建议分装后于 -20℃ 或 -80℃ 避光保存，避免反复冻融。-20℃ 可保存 1 个月，-80℃ 可保存半年。2. 荧光染料都存在淬灭的问题，染色后应尽快检测荧光强度，且整个实验过程尽量避光下进行。3. 为减缓荧光淬灭，可以使用抗荧光淬灭封片液 (MCE Cat. No.: HY-K1042)。4. 请根据实际情况调整 LDS-751 工作液浓度。5. 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品。6. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
Name	LDS-751
CAS	181885-68-7
Formula	C25H30ClN3O4
Molar Mass	471.98
Appearance	Solid
Transport	Room temperature in continental US; may vary elsewhere.
Storage	-20°C, sealed storage, away from moisture and light *该产品在溶液状态不稳定，建议您现用现配，即刻使用。
Reference	[1]. Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123(1):103-12. [2]. Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123(1):103-12. [3]. Terstappen LW, et al. Five-dimensional flow cytometry as a new approach for blood and bone marrow differentials. Cytometry. 1988;9(6):548-556. [4]. McCarthy DA, et al. A simple flow cytometric procedure for the determination of surface antigens on unfixed leucocytes in whole blood. J Immunol Methods. 1993;163(2):155-160.

PeptideDB

LDS-751

CAS: 181885-68-7 F: C25H30ClN3O4 W: 471.98