| Bioactivity | L-Leucine is an essential branched-chain amino acid (BCAA), which activates the mTOR signaling pathway[1]. | ||||||||||||
| Invitro | L-Leucine (10 mM) treatment impairs endocrine progenitor cell development[1].In E13.5 rat pancreatic explants, in absence of added L-Leucine, Ngn3 mRNA levels increased after 1 day of culture, peaked on day 3, and then decreased. When L-Leucine is added, a dramatic decrease is observed in Ngn3 mRNA levels. This decrease in Ngn3 mRNA levels was paralleled by a decrease in the number of Ngn3-expressing cells (4728±408 vs. 959±28; PNgn3, its target Insm1, and insulin[1]. Leucine stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing mTORC1 activation. L-Leucine increases intracellular HIF-1α levels and activates the HIF-1α signaling pathway, and these two effects are mediated by the mTOR signaling pathway. This process results in Ngn3 repression and, consequently, decreases β-cell differentiation[1].L-leucine stimulates mTORC1 by through a mechanism that involves the leucyl tRNA synthase promoting the activity of GTP activating proteins that act on mTORC1[2]. | ||||||||||||
| Name | L-Leucine | ||||||||||||
| CAS | 61-90-5 | ||||||||||||
| Formula | C6H13NO2 | ||||||||||||
| Molar Mass | 131.17 | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
|