| Bioactivity | HyT36 is a low molecular weight hydrophobic tag that promotes the degradation of fusion protein and pseudokinase Her3[1][2][3]. | ||||||||||||
| Invitro | HyT36 (10 µM；24 h) 降低表达 GFP-HaloTag2/Fz4-HaloTag2/GFP-HaloTag7 融合蛋白的 HEK293 细胞中 GFP-HaloTag2/Fz4-HaloTag2 的表达[1]。HyT36 (10 µM；2 h 或 48 h) 降低了表达 HA-EGFP-HaloTag2 的 HEK293 Flp-In 细胞中 EGFP 的表达，加强了 Hsp70 与 HA-EGFP-HaloTag2 融合蛋白的结合[3]。HyT36 (50 nM；24 h) 降低了表达 HA-EGFP-HaloTag2 的 HEK293 Flp-In 细胞中 EGFP-HaloTag2 的含量[3]。HyT36 (1 µM；4 d) 降低了表达 HA−HaloTag2−HRasG12V 的 NIH-3T3 细胞中 HA−HaloTag2−HRasG12V 的表达[3]。 Western Blot Analysis[1][3] Cell Line: | ||||||||||||
| Name | HyT36 | ||||||||||||
| CAS | 1323151-45-6 | ||||||||||||
| Formula | C25H44ClNO3 | ||||||||||||
| Molar Mass | 442.07 | ||||||||||||
| Appearance | Oil | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. Tae HS, et al. Identification of hydrophobic tags for the degradation of stabilized proteins. Chembiochem. 2012 Mar 5;13(4):538-41. [2]. Rubner S, et al. Poloxin-2HT+: changing the hydrophobic tag of Poloxin-2HT increases Plk1 degradation and apoptosis induction in tumor cells. Org Biomol Chem. 2019 Mar 20;17(12):3113-3117. [3]. Neklesa TK, et al. A bidirectional system for the dynamic small molecule control of intracellular fusion proteins. ACS Chem Biol. 2013 Oct 18;8(10):2293-2300. |