| Bioactivity | Diphenylterazine (DTZ) is a bioluminescence agent. Diphenylterazine alone yielded very little background, leading to excellent signal-to-background ratios[1]. Storage: protect from light. |
| Invitro | Diphenylterazine elicits minimal cell toxicity at millimolar concentrations[1].Notes: To make a stock solution for DTZ (Diphenylterazine), first, a premixture is prepared by dissolving 17.6 mg of L-ascorbic acid (HY-B0166) in 10 mL ethanol and 10 mL 1,2-propanediol; next, 1 mg of DTZ is dissolved in 88 µL of the premix, resulting in a 30 mM DTZ stock solution containing 5 mM L-ascorbic acid[3]. |
| In Vivo | Diphenylterazine injections into untransfected BALB/c mice do not yield any background emission. The bioluminescence resulting from intraperitoneally injected Diphenylterazine displays extended kinetics[1].DTZ (0.3 μmol/mouse (1.13 mg.ml-1/100 ul/mouse); i.v.) treatment can track tumor growth in a xenograft NU/J mouse model[2]. |
| Name | Diphenylterazine |
| CAS | 344940-63-2 |
| Formula | C25H19N3O |
| Molar Mass | 377.44 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -80°C, protect from light, stored under nitrogen |
| Reference | [1]. Yeh HW, et al. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974. [2]. Hsien-Wei Yeh, et al. ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. ACS Chem Biol. 2019 May 17;14(5):959-965. [3]. Practical Notes for teLuc-DTZ and Antares2-DTZ (updated 07/29/2019). |
Diphenylterazine
CAS: 344940-63-2 F: C25H19N3O W: 377.44
Diphenylterazine (DTZ) is a bioluminescence agent. Diphenylterazine alone yielded very little background, leading to exc
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