Acridine Orange hydrochloride_product_DataBase

Bioactivity	Acridine Orange hydrochloride is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange hydrochloride produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3].
Invitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[4].1. 用 Acridine Orange hydrochloride 染色细胞 (1 μM; 20 分钟; 37 ℃)。2. 用 PBS 洗涤细胞。3. 通过共聚焦激光扫描显微镜 (FV3000，Olympus) 观察细胞。
In Vivo	Acridine Orange hydrochloride (0.1 mg/kg，静脉注射，狗) 在 30 天内没有表现出临床毒性迹象，血液中也没有发现异常[1]。
Name	Acridine Orange hydrochloride
CAS	65-61-2
Formula	C17H20ClN3
Molar Mass	301.81
Appearance	Solid
Transport	Room temperature in continental US; may vary elsewhere.
Storage	4°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Reference	[1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8. [2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56. [3]. Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925. [4]. Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55(1):e13172.

PeptideDB

Acridine Orange hydrochloride

CAS: 65-61-2 F: C17H20ClN3 W: 301.81