| Bioactivity | BCECF-AM is a cell membrane permeable compound widely used as a fluorescent indicator for intracellular pH. Storage: protect from light. |
| Invitro | Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K+/H+ ionophor nigericin does not inhibit hydrogenosomal loading with BCECF-AM[1].The pH-sensitive fluorescent dyes to measure cytosolic pH.1.Prepare a 2 to 20 mM stock solution of BCECF-AM in DMSO. 2.Prepare a 5-50 µM BCECF-AM dye-loading solution in buffer solutions (HHBS or PBS).3. Add 1000 µL/well (6-well plate),100 µL/well (96-well plate) or 25 µL/well (384-well plate) BCECF-AM dye-loading solution into the cell plate.4. Incubate the dye-loading plate in a cell incubator for 30-60 minutes.5. Wash and replace the dye-loading solution with buffers.6. Run the pH assay by monitoring the fluorescence at Ex/Em = 490/535 nm or 430/535 nm for ratio measurements. |
| Name | BCECF-AM |
| CAS | 117464-70-7 |
| Formula | C40H36O19 |
| Molar Mass | 820.70 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, protect from light *该产品在溶液状态不稳定，建议您现用现配，即刻使用。 |
| Reference | [1]. Scott DA, et al. Analysis of the uptake of the fluorescent marker 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76(2):139-45. [2]. Clark Undem, et al. Endothelin-1 Augments Na+/H+ Exchange Activity in Murine Pulmonary Arterial Smooth Muscle Cells via Rho Kinase. PLoS One. 2012; 7(9): e46303. |