NBD-ε-aminocaproyl-Arg-Pro-Lys-Pro-Leu-Ala-Nva-Trp-Lys(DMACA)-NH2 Matrix Metalloproteinase Catalog

CAS	945414-97-1
Sequence	6-(7-Nitro-benzo[2,1,3]oxadiazol-4-ylamino)-hexanoyl-Arg-Pro-Lys-Pro-Leu-Ala-Nva-Trp-Lys((7-dimethylaminocoumarin-4-yl)-acetyl)-NH2
Sequence Single	NBD-ε-aminocaproyl-RPKPLA-Nva-WK(DMACA)-NH2
Molecular Formula	C78H111N21O16
Molecular Weight	1598.87
Technology	Synthetic
Storage	-20°C, avoid light, cool and dry place
Application	Cancer Research\|Inflammation Research
Description	NBD-ε-aminocaproyl-Arg-Pro-Lys-Pro-Leu-Ala-Nva-Trp-Lys(DMACA)-NH2 (NBD-RPKPLA-Nva-WK(DMC) amide) is an excellent fluorogenic substrate for hydrolysis of the matrix metalloproteinase stromelysin (MMP-3). This FRET substrate exhibits high sensitivity and improves accuracy at lower substrate turnover. It has a kcat/Km value of 2.14 x 10⁴ M-1s-1 and can be easily detected at 350 nm (excitation) and 465 nm (emission). Therefore this compound is a suitable tool for high-throughput inhibitor screening.
References	1. Purification of ADAM 10 from bovine spleen as a TNFalpha convertase. C.A.Lunn et al., FEBS Lett., 400, 333 (1997) 2. Critical role of glutamic acid 202 in the enzymatic activity of stromelysin-1 (MMP-3). B.Arza et al., Eur. J. Biochem., 268, 826 (2001) 3. Inactivation of the serpin alpha(2)-antiplasmin by stromelysin-1. H.R.Lijnen et al., Biochim. Biophys. Acta, 1547, 206 (2001)

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