| CAS | 202273-56-1 |
| Sequence | DABCYL-γ-Abu-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Glu(EDANS)-Ala-Lys-NH2 |
| Sequence Single | DABCYL-γ-Abu-RPKPVE-Nva-WRE(EDANS)AK-NH2 |
| Molecular Formula | C99H144N28O20S |
| Molecular Weight | 2078.48 |
| Synonyms | TNO003 |
| Technology | Synthetic |
| Storage | -20°C, avoid light, cool and dry place |
| Application | Cancer Research|Inflammation Research |
| Description | MMP Substrate IV also called TNO003, is a selective fluorogenic (FRET) matrix metalloproteinase (MMP) substrate. The kcat/Km value for MMP-3 (Stromelysin-1) is 29.000 M-1s-1, for MMP-13 13.000 M-1s-1, and for MMP-9 7.500 M-1s-1 (at pH 7.5, 25 °C). The fluorescence increase is monitored using an excitation wavelength of 360 nm and an emission wavelength of 490 nm. No activity against this substrate could be measured with MMP1, MMP-7, and MMP-8 (kcat/Km < 1000 M-1s-1). |
| References | 1. Highly increased levels of active stromelysin in rheumatoid synovial fluid determined by a selective fluorogenic assay. B Beekman, et al. FEBS Lett. 1997 Dec 1;418(3):305-9. |