MMP-1/MMP-9 Substrate, Fluorogenic Matrix Metalloproteinase Catalog

CAS	150956-92-6
Sequence	Dnp-Pro-β-cyclohexyl-Ala-Gly-Cys(Me)-His-Ala-Lys(N-Me-Abz)-NH2
Sequence Single	Dnp-P-β-cyclohexyl-AGC(Me)HAK(N-Me-Abz)-NH2
Molecular Formula	C49H68N14O12S
Molecular Weight	1077.23
Technology	Synthetic
Storage	-20°C, avoid light, cool and dry place
Application	Cancer Research\|Inflammation Research
Description	MMP-1/MMP-9 Substrate, Fluorogenic is a efficient fluorogenic substrate for two matrix metalloproteinases: interstitial collagenase (MMP-1) and gelatinase (MMP-9). MMP-1/MMP-9 Substrate has favorable solubility characteristics. Both enzymes cleave this substrate between Gly and Cys(Me) (Smc), liberating a cleavage product with a fluorescence signal suitable for inhibitor screening and determining Ki values. The major advantage of this FRET substrate is its adaptability to filters commonly available on commercial plate readers (excitation at 365 nm and emission at 450 nm).
References	1. Comparison of the hemoglobin adducts formed by administration of N-methylolacrylamide and acrylamide to rats. T.R.Fennell et al., Toxicol. Sci., 71, 164 (2003) 2. The role of ADAM 15 in glomerular mesangial cell migration. J.Martin et al., J. Biol. Chem., 277, 33683 (2002) 3. Method to analyze collagenase and gelatinase activity by fibroblasts in culture. L.J.Gould et al., In Vitro Cell. Dev. Biol. Anim., 35, 75 (1999)

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