| Sequence | (5,6)-FAM-Asp-Glu-Ala-Leu-Ala-Hyp-Tyr-Ile-Pro-Met-Asp-Asp-Asp-Phe-Gln-Leu-Arg-Ser-Phe-NH2 |
| Sequence Single | (5,6)-FAM-Asp-EALA-Hyp-YIPMDDDFQLRSF-NH2 |
| Molecular Formula | C122H158N24O39S |
| Molecular Weight | 2616.76 |
| Technology | Synthetic |
| Storage | -20°C, avoid light, cool and dry place |
| Description | FAM-DEALAHypYIPMDDDFQLRSF is a 5,6-carboxyfluorescein (FAM) labeled HIF-1α peptide. It binds with high affinity to von Hippel-Lindau (VHL) protein (KD = 3 nM). FAM-DEALAHypYIPMDDDFQLRSF can be used to assess ligand binding to VHL in a direct fluorescence polarization (FP) displacement assay. Excitation/emission maxima (λ) = 485/520 nm. This product is a longer peptide version of FAM-DEALA-Hyp-YIPD. |
| References | 1. HIF-1α peptide derivatives with modifications at the hydroxyproline residue as activators of HIF-1α. Ahn et al (2009). Bioorg.Med.Chem.Lett. 19 4403 PMID: 19515556 2. Targeting the von Hippel-Lindau E3 ubiquitin ligase using small molecules to disrupt the VHL/HIF-1a interaction. Buckley et al (2012). J.Am.Chem.Soc. 134 4465 PMID: 22369643 3. Identification and Characterization of Von Hippel-Lindau-Recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-Binding Kinase 1. Andrews et al (2018). J.Med.Chem. 61 583 PMID: 28692295 |