DABCYL-γ-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS HIV Peptides and HIV Protease Catalog

CAS	127134-13-8
Sequence	DABCYL-γ-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS
Sequence Single	DABCYL-Gaba-SQNYPIVQ-EDANS
Molecular Formula	C73H97N17O18S
Molecular Weight	1532.74
Technology	Synthetic
Storage	-20°C, avoid light, cool and dry place
Application	Infectious Disease
Description	DABCYL-γ-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is a fluorogenic HIV-1 protease substrate, it is consists of an octapeptide with a fluorescent donor (EDANS) and a quenching acceptor (DABCYL), attached at the COOH- and NH2-termini. The γ-Abu spacer was inserted to avoid potential steric hindrance of substrate binding by the bulky acceptor. This FRET substrate is cleaved by the HIV-1 protease at the Tyr-Pro bond which results in a time-dependent increase in fluorescence intensity; excitation at 340 nm, emission at 490 nm.
References	1. Activation of the herpes simplex virus type 1 protease. D.L.Hall and P.L.Darke, J. Biol. Chem., 270, 22697 (1995) 2. Molecular tongs containing amino acid mimetic fragments: new inhibitors of wild-type and mutated HIV-1 protease dimerization. L.Bannwarth et al., J. Med. Chem., 49, 4657 (2006) 3. Human immunodeficiency virus type 1 proteinase resistance to symmetric cyclic urea inhibitor analogs. U.Nillroth et al., Antimicrob. Agents Chemother., 41, 2383 (1997) 4. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer. E.D.Matayoshi et al., Science, 247, 954 (1990)

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