CAS | 142988-22-5 |
Sequence | DABCYL-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS |
Sequence Single | DABCYL-Abu-HPFHLVIHT-EDANS |
Molecular Formula | C90H120N22O16S |
Molecular Weight | 1798.15 |
Technology | Synthetic |
Storage | -20°C, avoid light, cool and dry place |
Application | Cardiovascular System & Diseases |
Description | DABCYL-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS is a fluorogenic peptide substrate, it has been developed to continuously measure the proteolytic activity of human renin (Km = 1.5 µM at physiological pH). Cleavage of the FRET substrate occurs specifically at the Leu-Val bond and corresponds to the renin cleavage site of angiotensinogen. By means of this assay as low as 30 ng/mL renin can be detected after an incubation of only 3-5 min. |