yGsy2p-IN-H23 is a potent inhibitor of yeast glycogen synthase 2 (yGsy2p) with IC50 of 875 µM for human glycogen synthase 1 (hGYS1). yGsy2p-IN-H23 binds within the uridine diphosphate glucose binding pocket of yGsy2p. yGsy2p-IN-H23 may be used in research related to glycogen storage diseases (GSDs).

Physicochemical Properties

Molecular Formula	C23H27N3O2
Molecular Weight	377.479385614395
Exact Mass	377.21
CAS #	1269190-98-8
PubChem CID	50985590
Appearance	Off-white to brown solid powder
LogP	3.8
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	6
Heavy Atom Count	28
Complexity	485
Defined Atom Stereocenter Count	0
InChi Key	YGPHAIXUDLLWHC-UHFFFAOYSA-N
InChi Code	InChI=1S/C23H27N3O2/c1-25-13-6-9-19(25)12-14-26-16-24-22(17-7-4-3-5-8-17)23(26)18-10-11-20(27)21(15-18)28-2/h3-5,7-8,10-11,15-16,19,27H,6,9,12-14H2,1-2H3
Chemical Name	2-methoxy-4-[3-[2-(1-methylpyrrolidin-2-yl)ethyl]-5-phenylimidazol-4-yl]phenol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: 875 µM (hGYS1)[1]
ln Vitro	yGsy2p-IN-H23 (compound H23) has IC50 values of 161 μM and 875 μM against hGYS1\~634S8, 11N, or wild-type hGYS1. In the absence of G6P, the yGsy2p-IN-H23's IC50 values against yeast and human glycogen synthase (GS) were 280 μM and 263 μM, respectively. G6P is not directly competed with by yGsy2p-IN-H23 [1].
References	[1]. Discovery and Development of Small-Molecule Inhibitors of Glycogen Synthase. J Med Chem. 2020 Apr 9;63(7):3538-3551.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (264.91 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.43 mg/mL (3.79 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1.43 mg/mL (3.79 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1.43 mg/mL (3.79 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6491 mL	13.2457 mL	26.4915 mL
	5 mM	0.5298 mL	2.6491 mL	5.2983 mL
	10 mM	0.2649 mL	1.3246 mL	2.6491 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.