tCFA15 is a trimethylcyclohexene long-chain fatty alcohol with 15 carbon atoms in the side chain. It can promote the differentiation of nerve cells and may be used to regulate the Notch signaling pathway.

Physicochemical Properties

Molecular Formula	C24H44O2
Molecular Weight	364.60496
Exact Mass	364.334
CAS #	220757-88-0
PubChem CID	9799188
Appearance	Light yellow to yellow ointment
LogP	7.145
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	15
Heavy Atom Count	26
Complexity	420
Defined Atom Stereocenter Count	0
SMILES	O=C1C(C)=C(CCCCCCCCCCCCCCCO)C(C)(C)CC1
InChi Key	FGMAOXGOTRUOKJ-UHFFFAOYSA-N
InChi Code	InChI=1S/C24H44O2/c1-21-22(24(2,3)19-18-23(21)26)17-15-13-11-9-7-5-4-6-8-10-12-14-16-20-25/h25H,4-20H2,1-3H3
Chemical Name	3-(15-hydroxypentadecyl)-2,4,4-trimethylcyclohex-2-en-1-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	tCFA15 (10 nM-1 μM) stimulates neuronal development at the expense of astrocytes via Notch1, which has a dose-dependent effect on the differentiation of neural stem cell-derived neurospheres. Notch1 mRNA levels in spheroids are selectively decreased by tCFA15 (1 μM), and this effect is similarly seen in lower Notch1 expression in cultures of neurons and glia [1]. Neurohypophyseal nerve terminals secrete arginine vasopressin when tCFA15 is present [2].
References	[1]. tCFA15, a trimethyl cyclohexenonic long-chain fatty alcohol, affects neural stem fate and differentiation by modulating Notch1 activity. Eur J Pharmacol. 2013 Oct 15;718(1-3):383-92. [2]. 3-(15-Hydroxypentadecyl)-2,4,4-trimethyl-2-cyclohexen-1-one and its effect on neuropeptide secretion. Bioorg Med Chem Lett. 2000 Nov 20;10(22):2537-9.
Additional Infomation	2-Cyclohexen-1-one, 3-(15-hydroxypentadecyl)-2,4,4-trimethyl- is a long-chain fatty alcohol.

Solubility Data

Solubility (In Vitro)

DMSO : ~130 mg/mL (~356.56 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.17 mg/mL (5.95 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.17 mg/mL (5.95 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7427 mL	13.7137 mL	27.4273 mL
	5 mM	0.5485 mL	2.7427 mL	5.4855 mL
	10 mM	0.2743 mL	1.3714 mL	2.7427 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.