rel-Cleroindicin F (Rengyolone) is a cyclohexylacetyl compound isolated from the fruit of Forsythia suspensa with anti-inflammatory activity. rel-Cleroindicin F has a strong inhibitory effect on the production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α). rel-Cleroindicin F inhibits the expression of inducible nitric oxide synthase (NO Synthase) and nitric oxide production by downregulating NF-κB and NF-κB kinase activities in LPS-stimulated RAW 264.7 cells.

Physicochemical Properties

Molecular Formula	C8H10O3
Molecular Weight	154.16
CAS #	94535-01-0
Related CAS #	Cleroindicin F;189264-47-9
SMILES	O[C@@]12CCO[C@@H]1CC(C=C2)=O
Synonyms	Rengyolone
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Rengyolone inhibits inducible nitric oxide synthase expression and nitric oxide production by down-regulation of NF-kappaB and p38 MAP kinase activity in LPS-stimulated RAW 264.7 cells. Biochem Pharmacol. 2006 Apr 14;71(8):1198-205.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.4868 mL	32.4338 mL	64.8677 mL
	5 mM	1.2974 mL	6.4868 mL	12.9735 mL
	10 mM	0.6487 mL	3.2434 mL	6.4868 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.