p-MPPI HCl is a selective, high-affinity 5-HT1A receptor blocker (antagonist). p-MPPI HCl can cross the BBB (blood-brain barrier) and has significant antidepressant and anxiolytic (anti-anxiety) effects.

Physicochemical Properties

Molecular Formula	C25H27N4O2I.HCL
Molecular Weight	578.87242
Exact Mass	578.095
CAS #	220643-77-6
Related CAS #	220643-77-6 (HCl);155204-23-2;
PubChem CID	11957651
Appearance	White to off-white solid powder
Boiling Point	657.5ºC at 760 mmHg
Flash Point	351.4ºC
Vapour Pressure	3.66E-17mmHg at 25°C
LogP	4.968
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	7
Heavy Atom Count	33
Complexity	578
Defined Atom Stereocenter Count	0
SMILES	COC1=CC=CC=C1N2CCN(CC2)CCN(C3=CC=CC=N3)C(=O)C4=CC=C(C=C4)I.Cl
InChi Key	PBPBIDMIRMWUCI-UHFFFAOYSA-N
InChi Code	InChI=1S/C25H27IN4O2.ClH/c1-32-23-7-3-2-6-22(23)29-17-14-28(15-18-29)16-19-30(24-8-4-5-13-27-24)25(31)20-9-11-21(26)12-10-20;/h2-13H,14-19H2,1H3;1H
Chemical Name	4-iodo-N-[2-[4-(2-methoxyphenyl)piperazin-1-yl]ethyl]-N-pyridin-2-ylbenzamide;hydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	p-MPPI hydrochloride exhibits no agonist action in presynaptic and postsynaptic 5-HT1A receptor activity tests conducted in vitro and in vivo [1].
ln Vivo	In the elevated plus maze, mice treated with p-MPPI hydrochloride (0.5-4.5 mg/kg; intraperitoneal) exhibit anxiolytic-like effects [2].
Animal Protocol	Animal/Disease Models: Adult male Swiss Webster mice (8-9 weeks) [2] Doses: 0.5, 1.5, 4.5 mg/kg Route of Administration: intraperitoneal (ip) injection Experimental Results: Significant and significant effects on conventional (open arm avoidance) Dose-related anxiolytic effects and behavioral (risk assessment) measures.
References	[1]. Sørensen E, et al. The selective 5-HT(1A) receptor antagonist p-MPPI antagonizes sleep--waking and behavioural effects of 8-OH-DPAT in rats. Behav Brain Res. 2001 Jun;121(1-2):181-7. [2]. Cao BJ, et al. Anxiolytic-like profile of p-MPPI, a novel 5HT1A receptor antagonist, in the murine elevated plus-maze. Psychopharmacology (Berl). 1997 Feb;129(4):365-71.

Solubility Data

Solubility (In Vitro)

DMSO : ~17.86 mg/mL (~30.85 mM) H2O : ~3.33 mg/mL (~5.75 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.79 mg/mL (3.09 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 17.9 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.79 mg/mL (3.09 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 17.9 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1.79 mg/mL (3.09 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 17.9 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.7275 mL	8.6375 mL	17.2750 mL
	5 mM	0.3455 mL	1.7275 mL	3.4550 mL
	10 mM	0.1728 mL	0.8638 mL	1.7275 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.