mPEG-Mal (MW 750) is a PEG analogue used for thiol pegylation of protein molecules. Its maleimide group (-Mal) degrades in aqueous media and has been used in drug delivery studies.

Physicochemical Properties

Molecular Weight	750(Average)
Exact Mass	270.121
CAS #	724722-89-8
Related CAS #	mPEG-Mal;724722-89-8
PubChem CID	58005095
Appearance	White to off-white solid powder
Melting Point	48-52 °C
LogP	-0.5
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	9
Heavy Atom Count	19
Complexity	349
Defined Atom Stereocenter Count	0
InChi Key	JCTGVOWJPZBVHT-UHFFFAOYSA-N
InChi Code	InChI=1S/C12H18N2O5/c1-18-8-9-19-7-5-13-10(15)4-6-14-11(16)2-3-12(14)17/h2-3H,4-9H2,1H3,(H,13,15)
Chemical Name	3-(2,5-dioxopyrrol-1-yl)-N-[2-(2-methoxyethoxy)ethyl]propanamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Development of a spectrophotometric biphasic assay for the estimation of mPEG-maleimide in thiol PEGylation reaction mixtures. Chemical Engineering Communications, 2016, 203(11): 1464-1472.
Additional Infomation	See also: Methoxy peg maleimide (20000 MW) (annotation moved to).

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)