m3227G(5)ppp(5')m6Am is a special RNA molecular structure consisting of a 3227-methylguanine (m3227G) cap, a triphosphate group and an N6,2'-O-dimethyladenosine. m6Am is a reversible modification located on the first coding nucleotide near the 5' cap end of mRNA, which can affect the stability of mRNA.

Physicochemical Properties

Molecular Formula	C25H37N10O17P3
Molecular Weight	842.54
Related CAS #	m3227G(5)ppp(5')m6Am triammonium
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Reversible methylation of m6Am in the 5' cap controls mRNA stability. Nature. 2017 Jan 19;541(7637):371-375.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.1869 mL	5.9344 mL	11.8689 mL
	5 mM	0.2374 mL	1.1869 mL	2.3738 mL
	10 mM	0.1187 mL	0.5934 mL	1.1869 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.