m3227G(5)ppp(5')Am is a special mRNA molecular structure, which consists of a 3227-methylguanine (m3227G) cap, a triphosphate group and a 2'-O-methyladenosine. Am is a reversible modification located on the first coding nucleotide near the 5' cap end of mRNA, which can affect the stability of mRNA.

Physicochemical Properties

Molecular Formula	C24H35N10O17P3
Molecular Weight	828.51
CAS #	178422-14-5
Related CAS #	m3227G(5)ppp(5')Am triammonium
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Reversible methylation of m6Am in the 5' cap controls mRNA stability. Nature. 2017 Jan 19;541(7637):371-375.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.2070 mL	6.0349 mL	12.0699 mL
	5 mM	0.2414 mL	1.2070 mL	2.4140 mL
	10 mM	0.1207 mL	0.6035 mL	1.2070 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.