Physicochemical Properties
| Molecular Formula | C20H24O4 |
| Exact Mass | 328.167 |
| CAS # | 114127-24-1 |
| Related CAS # | 114127-24-1 |
| PubChem CID | 10381847 |
| Appearance | Typically exists as solid at room temperature |
| LogP | 5.2 |
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 4 |
| Rotatable Bond Count | 6 |
| Heavy Atom Count | 24 |
| Complexity | 388 |
| Defined Atom Stereocenter Count | 2 |
| SMILES | C[C@@H](CC1=CC2=C(C=C1)OCO2)[C@H](C)CC3=CC(=C(C=C3)O)OC |
| InChi Key | QDDILOVMGWUNGD-KGLIPLIRSA-N |
| InChi Code | InChI=1S/C20H24O4/c1-13(8-15-4-6-17(21)19(10-15)22-3)14(2)9-16-5-7-18-20(11-16)24-12-23-18/h4-7,10-11,13-14,21H,8-9,12H2,1-3H3/t13-,14+/m1/s1 |
| Chemical Name | 4-[(2R,3S)-4-(1,3-benzodioxol-5-yl)-2,3-dimethylbutyl]-2-methoxyphenol |
| Synonyms | erythro-Austrobailignan-6 |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | At 100 μM, erythro-Austrobailignan-6 reduces the activity of DNA apical enzyme I by about 50% [1]. It has been observed that erythro-Austrobailignan-6 (0-30 μM, 24 or 48 h) suppresses 4T-1 and MCF-7 cell swelling [1]. In 4T-1 and MCF-7 cells, erythro-Austrobailignan-6 (2-30 μM, 24 or 48 hours) has been shown to be induced [1]. When applied to 4T-1 and MCF-7 cells, erythro-Austrobailignan-6 (10 μM, 30-120 min) enhances 4T-1, whereas erythro-Austrobailignan-6 (5 and 10 μM, 24 h) lowers EGFR/HER2 and topolignans. levels of enzymes[1]. |
| ln Vivo | erythro-Austrobailignan-6 (chow, for 20 days) suppresses tumor growth in 4T-1 tumor-bearing mice [1]. |
| Cell Assay |
Cell viability assay [1] Cell Types: 4T-1 and MCF-7 cells Tested Concentrations: 0-30 μM Incubation Duration: 24 or 48 h Experimental Results: Dose-dependent inhibition of cell proliferation. Western Blot Analysis[1] Cell Types: 4T-1 and MCF-7 Cell Tested Concentrations: 100 μM Incubation Duration: 30, 60 or 120 minutes Experimental Results: Increased p38 and JNK phosphorylation. Activates caspase-3 and inhibits CDK-4 and cyclin D1. |
| Animal Protocol |
Animal/Disease Models: 4T-1 tumor-bearing mice [1] Doses: 20 mg/kg/day Route of Administration: Feeding Experimental Results: Inhibited tumor growth by 40.8%; Downregulation of CDK-4, cyclin D1, HER2 and integrin β3; Cleaved caspase-3 is upregulated in tumors. |
| References |
[1]. Erythro-austrobailignan-6 down-regulates HER2/EGFR/integrinβ3 expression via p38 activation in breast cancer. Phytomedicine. 2017 Jan 15;24:24-30. [2]. Inhibition of DNA topoisomerases I and II and cytotoxicity by lignans from Saururus chinensis. Arch Pharm Res. 2009 Oct;32(10):1409-15. |
| Additional Infomation | 4-[(2R,3S)-2,3-Dimethyl-4-(1,3-benzodioxole-5-yl)butyl]-2-methoxyphenol has been reported in Machilus thunbergii with data available. |
Solubility Data
| Solubility (In Vitro) | May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |