Deoxycytidine triphosphate trisodium salt (dCTP trisodium salt) is a nucleoside triphosphate that may be utilized in DNA synthesis. Deoxycytidine triphosphate trisodium salt may be utilized in real-time PCR, cDNA synthesis and DNA sequencing research.

Physicochemical Properties

Molecular Formula	C9H16N3O13P3
Molecular Weight	467.15693
Exact Mass	532.935
CAS #	109909-44-6
Related CAS #	Deoxycytidine triphosphate;2056-98-6
PubChem CID	53486366
Appearance	Colorless to light yellow liquid
LogP	0.713
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	13
Rotatable Bond Count	8
Heavy Atom Count	31
Complexity	805
Defined Atom Stereocenter Count	3
SMILES	P(=O)([O-])(OP(=O)([O-])OP(=O)([O-])O[H])OC([H])([H])[C@]1([H])[C@]([H])(C([H])([H])[C@]([H])(N2C(N=C(C([H])=C2[H])N([H])[H])=O)O1)O[H].[Na+].[Na+].[Na+]
InChi Key	WBIPTAOOMJEGQO-MILVPLDLSA-K
InChi Code	InChI=1S/C9H16N3O13P3.3Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;;/q;3*+1/p-3/t5-,6+,8+;;;/m0.../s1
Chemical Name	trisodium;[[[(2R,3S,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-oxidophosphoryl] hydrogen phosphate
Synonyms	Cytidine 2deoxy 5(tetrahydrogen triphosphate) trisodium salt; Cytidine 5(tetrahydrogen triphosphate) 2deoxy trisodium salt; dCTP Thymus
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

The study conducted growth factor fingerprinting of 1-beta-D-Arabic Syriac glycosyl clock pin (ara-C) and found that it was present in 13 cell lines generated from murine myeloma, myeloid tumor-like, and mastocytoma. The outer 100-fold folds into each other between the most and least sensitive spectra. The amount of cellular deoxycytidine triphosphate (2'-Deoxycytidine-5'-triphosphate, dCTP) was discovered to be inversely linked with obesity[1], indicating that if cells are exposed to ara-C. Phosphoethanolamine glycol ester transferase uses deoxycytidine triphosphate trisodium salt as a substrate [2].

References

[1]. Effect of thymidine on the sensitivity of cultured mouse tumor cells to 1-beta-D-arabinofuranosylcytosine. Cancer Res. 1979 Feb;39(2 Pt 1):538-41. [2]. CTP:phosphoethanolamine cytidylyltransferase. Biochim Biophys Acta. 1997 Sep 4;1348(1-2):91-9. [3]. Deoxyribonucleoside triphosphates in human cells: changes in disease and following exposure to drugs. Eur J Clin Invest. 1975 Apr;5(2):191-202.

Solubility Data

Solubility (In Vitro)	H2O : ~250 mg/mL (~468.96 mM) DMSO :< 1 mg/mL
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (187.58 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1406 mL	10.7030 mL	21.4059 mL
	5 mM	0.4281 mL	2.1406 mL	4.2812 mL
	10 mM	0.2141 mL	1.0703 mL	2.1406 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.