c-Fms-IN-8 (compound 4a) is a type 2 inhibitor of CSF-1R with IC50 of 9.1 nM.

Physicochemical Properties

Molecular Formula	C27H30N2O5
Molecular Weight	462.54
Exact Mass	462.215
CAS #	1255303-58-2
PubChem CID	59634061
Appearance	White to off-white solid powder
LogP	3.7
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	10
Heavy Atom Count	34
Complexity	620
Defined Atom Stereocenter Count	0
SMILES	C1(=CC(=CC=N1)OCCO)C(N1CC(C2=CC(=C(C=C2)OCC2=CC=C(C=C2)CC)OC)C1)=O
InChi Key	KVXLTGRHKNTTMZ-UHFFFAOYSA-N
InChi Code	InChI=1S/C27H30N2O5/c1-3-19-4-6-20(7-5-19)18-34-25-9-8-21(14-26(25)32-2)22-16-29(17-22)27(31)24-15-23(10-11-28-24)33-13-12-30/h4-11,14-15,22,30H,3,12-13,16-18H2,1-2H3
Chemical Name	[3-[4-[(4-ethylphenyl)methoxy]-3-methoxyphenyl]azetidin-1-yl]-[4-(2-hydroxyethoxy)pyridin-2-yl]methanone
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Discovery of a novel azetidine scaffold for colony stimulating factor-1 receptor (CSF-1R) Type II inhibitors by the use of docking models. Bioorg Med Chem Lett. 2019 Jan 1;29(1):115-118.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~216.20 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (5.40 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1620 mL	10.8099 mL	21.6198 mL
	5 mM	0.4324 mL	2.1620 mL	4.3240 mL
	10 mM	0.2162 mL	1.0810 mL	2.1620 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.