β-catenin-IN-2 is a potent β-catenin inhibitor, compound H1B1, disclosed in patent US20150374662A1. β-catenin-IN-2 may be used in colorectal cancer research.

Physicochemical Properties

Molecular Formula	C15H14FN3
Molecular Weight	255.290166378021
Exact Mass	255.117
CAS #	1458664-10-2
PubChem CID	60979451
Appearance	Pink to red solid powder
LogP	3.5
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	2
Heavy Atom Count	19
Complexity	302
Defined Atom Stereocenter Count	0
SMILES	FC1C=CC2=C(C=1)NC(C1C=CC(=CC=1)N(C)C)=N2
InChi Key	ALWZHAOGGMSYPG-UHFFFAOYSA-N
InChi Code	InChI=1S/C15H14FN3/c1-19(2)12-6-3-10(4-7-12)15-17-13-8-5-11(16)9-14(13)18-15/h3-9H,1-2H3,(H,17,18)
Chemical Name	4-(6-fluoro-1H-benzimidazol-2-yl)-N,N-dimethylaniline
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In HCT116 cells, β-catenin/Tcf-4 transcriptional activity is dose-dependently inhibited by β-catenin-IN-2 (10–40 μM) [1].
References	[1]. Inhibitors of beta-catenin in treatment of colorectal cancer. Patent US20150374662A1.

Solubility Data

Solubility (In Vitro)

DMSO: 83.33 mg/mL (326.41 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (8.15 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.9171 mL	19.5856 mL	39.1711 mL
	5 mM	0.7834 mL	3.9171 mL	7.8342 mL
	10 mM	0.3917 mL	1.9586 mL	3.9171 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.