β-Melanocyte Stimulating Hormone (MSH), human TFA, is a 22-mer peptide that is an endogenous melanocortin-4 receptor (MC4-R) agonist.

Physicochemical Properties

Molecular Formula	C120H175F3N34O37S
Molecular Weight	2774.94
Appearance	Solid powder
Synonyms	Beta-MSH (1-22) (human) TFA
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Melanocortin-4 receptor (MC4-R)[1]
ln Vitro	β-Melanocyte Stimulating Hormone (MSH), human (β-MSH) has high affinity at both human MC4-R transfected into CHO cells (Ki=11.4±0.4 nM) and MC4-R in rat hypothalamic homogenates (Ki=5.0±0.4 nM)[1].
References	[1]. beta-MSH: a functional ligand that regulated energy homeostasis via hypothalamic MC4-R? Peptides. 2003 Mar;24(3):397-405.

Solubility Data

Solubility (In Vitro)	H2O : 100 mg/mL (36.04 mM; with sonication)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (36.04 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonocation. (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.3604 mL	1.8018 mL	3.6037 mL
	5 mM	0.0721 mL	0.3604 mL	0.7207 mL
	10 mM	0.0360 mL	0.1802 mL	0.3604 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.