β-CGRP, human TFA (Human β-CGRP TFA) is a calcitonin peptide that acts through the complex of calcitonin receptor-like receptor CRLR and RAMP. In the cell system, it affects CRLR/RAMP1 and CRLR The IC50s for /RAMP2 are 1 nM and 300 nM respectively.

Physicochemical Properties

Molecular Formula	C164H268F3N51O50S3
Molecular Weight	3907.38
Appearance	Typically exists as solid at room temperature
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: 1 nM (CRLR/RAMP1, cell assay), 300 nM (CRLR/RAMP2, cell assay)[1]
ln Vitro	Human β-CGRP is a calcitonin peptide that functions via a complex of calcitonin receptor-like receptor (CRLR) and receptor activity modifying protein (RAMP) with an IC50 of 1 nM. NG108-15 and HEK293T cells that express CRLR and RAMP2 express 130 nM and 300 nM, respectively [1]. SK-N-MC and Swiss 3T3 cells both express CRLR and RAMP1, and SK-N-MC and Swiss 3T3 cells express both CRLR and RAMP1 [1]. In addition to being a strong vasodilator, CGRP has anti- and pro-inflammatory properties [2].
References	[1]. RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor. Nature. 1998 May 28;393(6683):333-9. [2]. Calcitonin gene-related peptide: physiology and pathophysiology. Physiol Rev. 2014 Oct;94(4):1099-142.

Solubility Data

Solubility (In Vitro)	H2O :~100 mg/mL (~25.59 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 50 mg/mL (12.80 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.2559 mL	1.2796 mL	2.5593 mL
	5 mM	0.0512 mL	0.2559 mL	0.5119 mL
	10 mM	0.0256 mL	0.1280 mL	0.2559 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.