Zinquin ethyl ester is a fluorescent analogue of Zinquin and a fluorescent probe for cellular zinc. Zinquin ethyl ester is able to penetrate cell membranes and is lipophilic and zinc-sensitive. Zinquin ethyl ester can combine with Zn2+ in the presence of Ca2+ and Mg2+ to produce blue fluorescence. .

Physicochemical Properties

Molecular Formula	C21H22N2O5S
Molecular Weight	414.47478
Exact Mass	414.124
CAS #	181530-09-6
PubChem CID	3638243
Appearance	White to off-white solid powder
Density	1.3±0.1 g/cm3
Boiling Point	578.0±60.0 °C at 760 mmHg
Flash Point	303.3±32.9 °C
Vapour Pressure	0.0±1.6 mmHg at 25°C
Index of Refraction	1.619
LogP	5.08
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	8
Heavy Atom Count	29
Complexity	642
Defined Atom Stereocenter Count	0
InChi Key	KCASTCXJTDRDFT-UHFFFAOYSA-N
InChi Code	InChI=1S/C21H22N2O5S/c1-4-27-20(24)13-28-17-11-16-8-7-15(3)22-21(16)19(12-17)23-29(25,26)18-9-5-14(2)6-10-18/h5-12,23H,4,13H2,1-3H3
Chemical Name	ethyl 2-[2-methyl-8-[(4-methylphenyl)sulfonylamino]quinolin-6-yl]oxyacetate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Zinc inhibits doxorubicin-activated calcineurin signal transduction pathway in H9c2 embryonic rat cardiac cells. Exp Biol Med (Maywood). 2007 May;232(5):682-9.

Solubility Data

Solubility (In Vitro)

DMSO : ≥ 50 mg/mL (~120.64 mM) H2O : < 0.1 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: 5 mg/mL (12.06 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4127 mL	12.0636 mL	24.1272 mL
	5 mM	0.4825 mL	2.4127 mL	4.8254 mL
	10 mM	0.2413 mL	1.2064 mL	2.4127 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.