Z433927330 is a potent and selective inhibitor of Aquaporin-7 (AQP7), less potently inhibits AQP3 and AQPs9, with IC50s of ~0.2 µM, ~0.7 µM and ~1.1 µM for mAQP7, mAQP3 and mAQP9, respectively.

Physicochemical Properties

Molecular Formula	C20H20N4O3
Molecular Weight	364.3978
Exact Mass	364.153
CAS #	1005883-72-6
PubChem CID	51116434
Appearance	White to off-white solid powder
LogP	2.6
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	7
Heavy Atom Count	27
Complexity	486
Defined Atom Stereocenter Count	0
SMILES	O(C([H])([H])C([H])([H])[H])C(C1C([H])=C([H])C(=C([H])C=1[H])N([H])C(N([H])C([H])([H])C1C([H])=C([H])C(=C([H])C=1[H])N1C([H])=C([H])C([H])=N1)=O)=O
InChi Key	KQUKHINHCUELQL-UHFFFAOYSA-N
InChi Code	InChI=1S/C20H20N4O3/c1-2-27-19(25)16-6-8-17(9-7-16)23-20(26)21-14-15-4-10-18(11-5-15)24-13-3-12-22-24/h3-13H,2,14H2,1H3,(H2,21,23,26)
Chemical Name	ethyl 4-(3-(4-(1H-pyrazol-1-yl)benzyl)ureido)benzoate
Synonyms	Z433927330 Z-433927330 Z 433927330
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Z433927330 has an IC50 of roughly 0.6 μM and inhibits triglyceride permeability[1].
References	[1]. Identification and characterization of potent and selective aquaporin-3 and aquaporin-7 inhibitors. J Biol Chem. 2019 May 3;294(18):7377-7387.

Solubility Data

Solubility (In Vitro)

DMSO : ≥ 280 mg/mL (~768.39 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.33 mg/mL (6.39 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 23.3 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.33 mg/mL (6.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 23.3 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7442 mL	13.7212 mL	27.4424 mL
	5 mM	0.5488 mL	2.7442 mL	5.4885 mL
	10 mM	0.2744 mL	1.3721 mL	2.7442 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.