Physicochemical Properties
| Molecular Formula | C21H22N4O6 |
| Molecular Weight | 426.42258 |
| Exact Mass | 426.153 |
| CAS # | 65022-15-3 |
| PubChem CID | 16218813 |
| Sequence | Cbz-Gly-Pro-pNA |
| SequenceShortening | GP |
| Appearance | White to off-white solid powder |
| Density | 1.4±0.1 g/cm3 |
| Boiling Point | 738.7±60.0 °C at 760 mmHg |
| Flash Point | 400.5±32.9 °C |
| Vapour Pressure | 0.0±2.4 mmHg at 25°C |
| Index of Refraction | 1.639 |
| LogP | 3 |
| Hydrogen Bond Donor Count | 2 |
| Hydrogen Bond Acceptor Count | 6 |
| Rotatable Bond Count | 7 |
| Heavy Atom Count | 31 |
| Complexity | 653 |
| Defined Atom Stereocenter Count | 1 |
| SMILES | O=C(N1CCC[C@H]1C(NC2=CC=C([N+]([O-])=O)C=C2)=O)CNC(OCC3=CC=CC=C3)=O |
| InChi Key | UTXSFKPOIVELPQ-SFHVURJKSA-N |
| InChi Code | InChI=1S/C21H22N4O6/c26-19(13-22-21(28)31-14-15-5-2-1-3-6-15)24-12-4-7-18(24)20(27)23-16-8-10-17(11-9-16)25(29)30/h1-3,5-6,8-11,18H,4,7,12-14H2,(H,22,28)(H,23,27)/t18-/m0/s1 |
| Chemical Name | benzyl N-[2-[(2S)-2-[(4-nitrophenyl)carbamoyl]pyrrolidin-1-yl]-2-oxoethyl]carbamate |
| Synonyms | Z-Gly-Pro-PNA; 65022-15-3; N-CBZ-Glycyl-L-proline 4-nitroanilide; Z-Gly-Pro-4-nitroanilide; MFCD00038815; benzyl N-[2-[(2S)-2-[(4-nitrophenyl)carbamoyl]pyrrolidin-1-yl]-2-oxoethyl]carbamate; Benzyl (S)-(2-(2-((4-nitrophenyl)carbamoyl)pyrrolidin-1-yl)-2-oxoethyl)carbamate; SCHEMBL562368; |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| Targets |
- Prolyl endopeptidase (PEP)：Z-Gly-Pro-pNA is a synthetic substrate for PEP, designed to be cleaved by the enzyme at the Pro-pNA bond. The cleavage releases p-nitroaniline (pNA), which can be quantified spectrophotometrically to measure PEP activity. No inhibitory activity or binding constants (IC50/Ki) were reported for Z-Gly-Pro-pNA itself. [1] |
| Enzyme Assay |
- Prolyl endopeptidase activity assay：
1. Z-Gly-Pro-pNA (0.5 mM) is dissolved in buffer containing Tris-HCl (50 mM, pH 7.5), NaCl (100 mM), and dithiothreitol (1 mM).
2. The reaction mixture is incubated with PEP (0.1-0.5 μg/mL) at 37°C for 30 minutes.
3. The reaction is terminated by adding acetic acid (10%), and the absorbance at 405 nm is measured to quantify released pNA.
4. The enzyme activity is calculated based on a standard curve of pNA. [1] |
| References |
[1]. Prolyl Endopeptidase Inhibitory Activity of 6-O-Palmitoyl L-Ascorbic Acid. Journal of Applied Biological Chemistry, 2006, 49(3): 110-113. |
| Additional Infomation |
- Role in research：Z-Gly-Pro-pNA is used as a chromogenic substrate to assay PEP activity in biochemical studies. It allows for quantitative assessment of enzyme inhibition by compounds like 6-O-palmitoyl L-ascorbic acid, which was tested in the referenced study. [1] - Limitations：As a substrate, Z-Gly-Pro-pNA does not exhibit pharmacological activity or therapeutic effects. Its use is restricted to in vitro enzyme assays. [1] |
Solubility Data
| Solubility (In Vitro) | May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3451 mL | 11.7255 mL | 23.4511 mL | |
| 5 mM | 0.4690 mL | 2.3451 mL | 4.6902 mL | |
| 10 mM | 0.2345 mL | 1.1726 mL | 2.3451 mL |