Y29 is a potent PFKFB3 inhibitor (antagonist) with IC50 of 0.183 µM and a competitive inhibitor of Fru-6-P with Ki of 0.094 µM. Y29 also inhibits HeLa cell growth with a GI50 of 2.7 µM.

Physicochemical Properties

Molecular Formula	C12H10O5
Molecular Weight	234.2048
Exact Mass	234.052
CAS #	6093-71-6
PubChem CID	5289613
Appearance	White to off-white solid powder
Density	1.4±0.1 g/cm3
Boiling Point	448.3±45.0 °C at 760 mmHg
Melting Point	170.0 to 174.0 °C
Flash Point	178.7±22.2 °C
Vapour Pressure	0.0±1.1 mmHg at 25°C
Index of Refraction	1.598
LogP	1.85
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	17
Complexity	360
Defined Atom Stereocenter Count	0
SMILES	O1C(C(C(=O)OC([H])([H])C([H])([H])[H])=C([H])C2C([H])=C([H])C(=C([H])C1=2)O[H])=O
InChi Key	IETDBZQIWIJQJG-UHFFFAOYSA-N
InChi Code	InChI=1S/C12H10O5/c1-2-16-11(14)9-5-7-3-4-8(13)6-10(7)17-12(9)15/h3-6,13H,2H2,1H3
Chemical Name	ethyl 7-hydroxy-2-oxochromene-3-carboxylate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Solubility Data

Solubility (In Vitro)

DMSO : ~25 mg/mL (~106.75 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (10.67 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.2699 mL	21.3493 mL	42.6985 mL
	5 mM	0.8540 mL	4.2699 mL	8.5397 mL
	10 mM	0.4270 mL	2.1349 mL	4.2699 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.