YF-2 is a selective, BBB (blood-brain barrier) permeable/penetrable histone acetyltransferase agonist that can acetylate H3 in the hippocampus. The EC50s for CBP, PCAF and GCN5 are 2.75 μM respectively. , 29.04 μM and 49.31 μM, have no effect on HDAC. Has anti-tumor and anti-Alzheimer's activity.

Physicochemical Properties

Molecular Formula	C₂₀H₂₂CLF₃N₂O₃
Molecular Weight	430.85
Exact Mass	430.127
CAS #	1311423-89-8
Related CAS #	YF-2 hydrochloride;1312005-62-1
PubChem CID	53230697
Appearance	Light yellow to yellow solid powder
LogP	5.334
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	8
Heavy Atom Count	29
Complexity	522
Defined Atom Stereocenter Count	0
InChi Key	TZHLDFAKTWDTOW-UHFFFAOYSA-N
InChi Code	InChI=1S/C20H22ClF3N2O3/c1-4-28-16-6-5-7-17(29-11-10-26(2)3)18(16)19(27)25-13-8-9-15(21)14(12-13)20(22,23)24/h5-9,12H,4,10-11H2,1-3H3,(H,25,27)
Chemical Name	N-[4-chloro-3-(trifluoromethyl)phenyl]-2-[2-(dimethylamino)ethoxy]-6-ethoxy-benzamide
Synonyms	YF2 YF-2 YF 2
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	YF-2 (0.03, 0.1, 0.25, 0.5, 1, 2.5, 5, 15, 40 and 80 μM, 72 hours) suppresses the swelling of U251, CCRF-CEM, Hs578T, NCI-ADR-RES cells [1].
ln Vivo	Under 20 mg/kg simulation, YF-2 (20 mg/kg, 2 hours before shock or 5 mg/kg, 30 minutes before shock, ip) has no influence on the model's model memory and can fix flaws in the simulation memory [ 1].
References	[1]. HISTONE ACETYLTRANSFERASE ACTIVATORS AND USES THEREOF. WO 2011072243A1.

Solubility Data

Solubility (In Vitro)

DMSO : ≥ 125 mg/mL (~290.12 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (4.83 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.08 mg/mL (4.83 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (4.83 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.3210 mL	11.6050 mL	23.2099 mL
	5 mM	0.4642 mL	2.3210 mL	4.6420 mL
	10 mM	0.2321 mL	1.1605 mL	2.3210 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.