Wnt pathway activator 2 is a potent Wnt activator with EC50 of 13 nM. For more details, check and find patent WO2012024404A1, compound 2.

Physicochemical Properties

Molecular Formula	C17H15NO4
Molecular Weight	297.31
Exact Mass	297.1
CAS #	1360540-82-4
PubChem CID	56833973
Appearance	Light yellow to yellow solid powder
LogP	1.8
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	5
Heavy Atom Count	22
Complexity	412
Defined Atom Stereocenter Count	0
SMILES	C(C1=CC=C2OCCOC2=C1)(=O)CCC(C1=NC=CC=C1)=O
InChi Key	DMBCGEJVWBLNDP-UHFFFAOYSA-N
InChi Code	InChI=1S/C17H15NO4/c19-14(5-6-15(20)13-3-1-2-8-18-13)12-4-7-16-17(11-12)22-10-9-21-16/h1-4,7-8,11H,5-6,9-10H2
Chemical Name	1-(2,3-dihydro-1,4-benzodioxin-6-yl)-4-pyridin-2-ylbutane-1,4-dione
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Diketones and hydroxyketones as catenin signaling pathway activators. WO2012024404A1.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~336.35 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 5 mg/mL (16.82 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.3635 mL	16.8175 mL	33.6349 mL
	5 mM	0.6727 mL	3.3635 mL	6.7270 mL
	10 mM	0.3363 mL	1.6817 mL	3.3635 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.