WWL-70 is a novel, potent inhibitor of α/β-hydrolase domain 6 (ABHD6) with IC50 of 70 nM. α/β-hydrolase domain 6 is an enzyme which catalyzes the hydrolysis of 2-arachidonylglycerol.

Physicochemical Properties

Molecular Formula	C27H23N3O3
Molecular Weight	437.48982
Exact Mass	437.173
CAS #	947669-91-2
PubChem CID	17759121
Appearance	White to off-white solid powder
Density	1.2±0.1 g/cm3
Boiling Point	653.1±55.0 °C at 760 mmHg
Flash Point	348.8±31.5 °C
Vapour Pressure	0.0±2.0 mmHg at 25°C
Index of Refraction	1.634
LogP	3.82
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	7
Heavy Atom Count	33
Complexity	635
Defined Atom Stereocenter Count	0
InChi Key	QTWNORFUQILKJL-UHFFFAOYSA-N
InChi Code	InChI=1S/C27H23N3O3/c1-30(18-19-3-2-4-24(17-19)22-13-15-29-16-14-22)27(32)33-25-11-9-21(10-12-25)20-5-7-23(8-6-20)26(28)31/h2-17H,18H2,1H3,(H2,28,31)
Chemical Name	4'-carbamoyl-[1,1'-biphenyl]-4-yl methyl(3-(pyridin-4-yl)benzyl)carbamate
Synonyms	WWL 70 WWL70 WWL-70.
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	When compared to untreated cells, 2-arachidonoylglycerol (2-AG) rose by 20% an hour after WWL70 (10 μM) treatment. WWL70 totally prevented the rise in PGE2 brought on by lipopolysaccharide (LPS) at 1 or 10 μM. WWL70 also decreased the mRNA expression of LPS-enhanced mPGES-1 and mPGES-2. About 100 nM is the IC50 of WWL70, which inhibits PGE2 biosynthesis[2].
ln Vivo	Treatment with 10 mg/kg WWL70 considerably improved performance, while post-treatment with 5 mg/kg WWL70 showed little impact. In traumatic brain injury (TBI) mice, WWL70 therapy enhances motor coordination in a concentration-dependent way. The fall latency rose in rats given 5 mg/kg WWL70 on days 3 and 7, respectively, from 74.92±4.8 to 99.57±5.21 (p<0.01) and 87.32±4.42 to 100.14±3.56 (p<0.05). injuries in comparison to the TBI group of vehicles. Beginning on the first day following injury, motor coordination is improved by WWL70 at a therapeutic level of 10 mg/kg. TBI mice treated with WWL70 were able to consistently switch arms throughout Y-maze exploration (69.67±4.98%) [3].
References	[1]. A functional proteomic strategy to discover inhibitors for uncharacterized hydrolases. J Am Chem Soc. 2007 Aug 8;129(31):9594-5. [2]. WWL70 attenuates PGE2 production derived from 2-arachidonoylglycerol in microglia by ABHD6-independent mechanism. J Neuroinflammation. 2017 Jan 10;14(1):7. [3]. Selective inhibition of alpha/beta-hydrolase domain 6 attenuates neurodegeneration, alleviates blood brain barrier breakdown, and improves functional recovery in a mouse model of traumatic brain injury. J Neurotrauma. 2013 Apr 1;30(7).

Solubility Data

Solubility (In Vitro)

DMSO : ~17.33 mg/mL (~39.61 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (5.71 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.2 mg/mL (5.03 mM) in 5% DMSO + 40% PEG300 + 5% Tween80 + 50% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.2858 mL	11.4288 mL	22.8577 mL
	5 mM	0.4572 mL	2.2858 mL	4.5715 mL
	10 mM	0.2286 mL	1.1429 mL	2.2858 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.