WST-3 is a novel and highly sensitive tetrazolium reagent that is able to produce a water-soluble, colored formazan dye with reducing agents such as NADH.

Physicochemical Properties

Molecular Formula	C19H10IN6NAO10S2
Molecular Weight	696.3411
Exact Mass	695.884
CAS #	515111-36-1
PubChem CID	9810247
Appearance	Light yellow to green yellow solid powder
LogP	4.648
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	12
Rotatable Bond Count	3
Heavy Atom Count	39
Complexity	1070
Defined Atom Stereocenter Count	0
InChi Key	LKVNWZJLLRMMAS-UHFFFAOYSA-M
InChi Code	InChI=1S/C19H11IN6O10S2.Na/c20-11-1-3-12(4-2-11)23-21-19(15-7-6-14(37(31,32)33)10-18(15)38(34,35)36)22-24(23)16-8-5-13(25(27)28)9-17(16)26(29)30/h1-10H,(H-,31,32,33,34,35,36)/q+1/p-1
Chemical Name	Sodium 4-[2-(2,4-dinitrophenyl)-3-(4-iodophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate
Synonyms	WST-3 WST 3 WST3
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

: Wawra A, Friesl-Hanl W, Jäger A, Puschenreiter M, Soja G, Reichenauer T, Watzinger A. Investigations of microbial degradation of polycyclic aromatic hydrocarbons based on (13)C-labeled phenanthrene in a soil co-contaminated with trace elements using a plant assisted approach. Environ Sci Pollut Res Int. 2018 Mar;25(7):6364-6377.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~143.61 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (3.59 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.4361 mL	7.1804 mL	14.3608 mL
	5 mM	0.2872 mL	1.4361 mL	2.8722 mL
	10 mM	0.1436 mL	0.7180 mL	1.4361 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.