VU6015929 is a potent, selective, orally bioactive DDR1/2 inhibitor (antagonist) with IC50 of 4.67 nM and 7.39 nM respectively. VU6015929 effectively blocks collagen-induced DDR1 activation and type IV collagen production.

Physicochemical Properties

Molecular Formula	C24H19F4N5O2
Molecular Weight	485.4336
Exact Mass	485.147
CAS #	2442597-56-8
PubChem CID	145925694
Appearance	White to yellow solid powder
Density	1.37±0.1 g/cm3(Predicted)
Boiling Point	553.3±50.0 °C(Predicted)
LogP	4.4
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	9
Rotatable Bond Count	7
Heavy Atom Count	35
Complexity	699
Defined Atom Stereocenter Count	0
InChi Key	IZKVIUJIUTXIRH-UHFFFAOYSA-N
InChi Code	InChI=1S/C24H19F4N5O2/c1-33-8-7-21(32-33)17-9-15(12-29-14-17)13-30-22-10-16(5-6-20(22)25)23(34)31-18-3-2-4-19(11-18)35-24(26,27)28/h2-12,14,30H,13H2,1H3,(H,31,34)
Chemical Name	4-fluoro-3-[[5-(1-methylpyrazol-3-yl)pyridin-3-yl]methylamino]-N-[3-(trifluoromethoxy)phenyl]benzamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The HEK293-DDR1b cells treated with VU6015929 (Compound 7e; 4-100 nM; 24 hours) showed a dose-dependent inhibition of collagen I-induced DDR1 phosphorylation. According to a study of the phosphorylated DDR1/total DDR1 ratio, VU6015929's IC50 is 0.7078 nM [1].
ln Vivo	VU6015929 (Compound 7e) was further examined in a rat IV (0.5 mg/kg)/PO (3 mg/kg) PK trial in 10% EtOH/40% PEG400/50% saline vehicle. VU6015929 demonstrates good in vitro:in vivo correlation (IVIC), has moderate in vivo clearance (CLp = 34.2 mL/min/kg), a half-life of approximately 3 hours, and a moderate volume of distribution at steady state (Vss = 4.3 L) /kg) and 12.5% oral bioavailability with a quick Tmax (0.75 hours) [1].
Cell Assay	Western Blot Analysis[1] Cell Types: HEK293-DDR1b Cell Tested Concentrations: 4 nM, 20 nM, 100 nM Incubation Duration: 24 hrs (hours) Experimental Results: Inhibits collagen I-induced DDR1 phosphorylation in a dose-dependent manner. Dramatically inhibits the production of type IV collagen.
References	[1]. Discovery of VU6015929: A Selective Discoidin Domain Receptor 1/2 (DDR1/2) Inhibitor to Explore the Role of DDR1 in Antifibrotic Therapy. ACS Med. Chem. Lett. 2019.

Solubility Data

Solubility (In Vitro)

DMSO : ~250 mg/mL (~515.01 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (4.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.28 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0600 mL	10.3001 mL	20.6003 mL
	5 mM	0.4120 mL	2.0600 mL	4.1201 mL
	10 mM	0.2060 mL	1.0300 mL	2.0600 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.