Usmarapride (SUVN-D4010) is a specific, orally bioactive and BBB (blood-brain barrier) permeable (penetrable) 5-HT4 receptor partial agonist (EC50=nM). Usmarapride (SUVN-D4010) may be utilized to study cognitive deficits associated with AD/Alzheimer's disease.

Physicochemical Properties

Molecular Formula	C23H31N5O6
Molecular Weight	473.52
Exact Mass	473.227
CAS #	1428862-33-2
Related CAS #	Usmarapride free base;1428862-32-1
PubChem CID	71508290
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	10
Rotatable Bond Count	8
Heavy Atom Count	34
Complexity	557
Defined Atom Stereocenter Count	0
SMILES	O1C(C2C3=C([H])C([H])=C([H])C([H])=C3N(C([H])(C([H])([H])[H])C([H])([H])[H])N=2)=NN=C1C1([H])C([H])([H])C([H])([H])N(C([H])([H])C([H])([H])C([H])([H])OC([H])([H])[H])C([H])([H])C1([H])[H].O([H])C(C(=O)O[H])=O
InChi Key	XHXCJKNEBFAUSE-UHFFFAOYSA-N
InChi Code	InChI=1S/C21H29N5O2.C2H2O4/c1-15(2)26-18-8-5-4-7-17(18)19(24-26)21-23-22-20(28-21)16-9-12-25(13-10-16)11-6-14-27-3;3-1(4)2(5)6/h4-5,7-8,15-16H,6,9-14H2,1-3H3;(H,3,4)(H,5,6)
Chemical Name	2-[1-(3-methoxypropyl)piperidin-4-yl]-5-(1-propan-2-ylindazol-3-yl)-1,3,4-oxadiazole;oxalic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	5-HT4 Receptor 44 nM (EC50)
ln Vivo	In the object recognition test (ORT), usmarapride (SUVN-D4010) (1-3 mg/kg; po; male Wistar rats 10–12 weeks old) reduces the long-term memory deficits[1]. Scopolamine-induced amnesia is considerably reversed by usmarapride (1, 3, and 10 mg/kg; po)[1]. At 3.0 mg/kg, usmarapride has a statistically significant impact on the recognition index and exploration time[1]. Good oral exposures, good absorption, and good cerebral exposures are demonstrated by usmarapride (SUVN-D4010) in rats[1]. Pharmacokinetics of Usmarapride in Rats[1]: F% Cmax (ng/mL), AUC (ng· hr/mL), t1/2 (h), Vdss (L/kg), CL (mLg/min/kg) 34 360 709 1.7 8.0 76
References	[1]. Nirogi, Ramakrishna; Mohammed, Abdul Rasheed; Yarlgadda, Suresh; Ravella, Srinivasa Rao; Shinde, Anil Karbhari; Kambhampati, Ramasastri; Roayalley, Praveen Kumar; Jayarajan, Pradeep; Bhyrapuneni, Gopinadh; Patnala, Sriramachandra Murthy; et al. Preparatio

Solubility Data

Solubility (In Vitro)

DMSO: 33.33 mg/mL (70.39 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (5.28 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.28 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (5.28 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1118 mL	10.5592 mL	21.1184 mL
	5 mM	0.4224 mL	2.1118 mL	4.2237 mL
	10 mM	0.2112 mL	1.0559 mL	2.1118 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.