Physicochemical Properties
| Molecular Formula | C16H14N2O2 |
| Molecular Weight | 266.29456 |
| Exact Mass | 266.105 |
| CAS # | 168835-90-3 |
| PubChem CID | 2050 |
| Appearance | Light yellow to khaki solid powder |
| LogP | 2.9 |
| Hydrogen Bond Donor Count | 2 |
| Hydrogen Bond Acceptor Count | 4 |
| Rotatable Bond Count | 1 |
| Heavy Atom Count | 20 |
| Complexity | 338 |
| Defined Atom Stereocenter Count | 0 |
| SMILES | CC1=CC2=NC=C(N=C2C=C1C)C3=CC(=C(C=C3)O)O |
| InChi Key | SMKFYHOKXJUJOT-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C16H14N2O2/c1-9-5-12-13(6-10(9)2)18-14(8-17-12)11-3-4-15(19)16(20)7-11/h3-8,19-20H,1-2H3 |
| Chemical Name | 4-(6,7-dimethylquinoxalin-2-yl)benzene-1,2-diol |
| Synonyms | Tyrphostin AG1433; Tyrphostin AG-1433 |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | Treatment of glioblastoma cells with tyrophostin AG1433 (0.1-100 μM; 72 hours; GB8B cells) causes moderate cytotoxicity [1]. |
| ln Vivo | Angiogenesis was examined using the Chorioallantoic membrane (CAM) assay to evaluate the impact of Flk-i inhibitors. Tyrphostin AG1433 (SU1433) is suited for CAM in chicken embryos that are 4-6 days old and is made from methylcellulose particles. Under the particles, tyrosine phosphatase AG1433 inhibits the growth of new fish algae [2]. |
| Cell Assay |
Cell viability assay [1] Cell Types: GB8B Cell Tested Concentrations: 0.1 μM, 1 μM, 5 μM, 10 μM, 20 μM, 30 μM, 50 μM, 60 μM, 100 μM Incubation Duration: 72 hrs (hours) Experimental Results: Significant induction Cell death occurred in a concentration-dependent manner in GB8B cells. |
| References |
[1]. Silencing of epidermal growth factor, latrophilin and seven transmembrane domain-containing protein 1 (ELTD1) via siRNA-induced cell death in glioblastoma. J Immunoassay Immunochem. 2017;38(1):21-33. [2]. Flk-1 as a target for tumor growth inhibition. Cancer Res. 1996 Aug 1;56(15):3540-5. [3]. The ZFHX3 (ATBF1) transcription factor induces PDGFRB, which activates ATM in the cytoplasm to protect cerebellar neurons from oxidative stress. Dis Model Mech. 2010 Nov-Dec;3(11-12):752-62. [4]. The vascular endothelial growth factor receptor KDR activates multiple signal transduction pathways in porcine aortic endothelial cells. J Biol Chem. 1997 Dec 19;272(51):32521-7. |
| Additional Infomation | Tyrphostin AG 1433 is a member of the Tyrphostin family of tyrosine kinase inhibitors that selectively inhibits platelet-derived growth factor beta-receptor kinase and angiogenesis. (NCI) |
Solubility Data
| Solubility (In Vitro) | DMSO : ~62.5 mg/mL (~234.71 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.81 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7553 mL | 18.7765 mL | 37.5530 mL | |
| 5 mM | 0.7511 mL | 3.7553 mL | 7.5106 mL | |
| 10 mM | 0.3755 mL | 1.8777 mL | 3.7553 mL |