Tubulin inhibitor 6 (Compound 14b) is a novel and potent tubulin inhibitor (mitotic inhibitor or tubulin destabilizer) with anticancer activity. Tubulin polymerization is inhibited with an IC50 of 0.87 μM. K562 cell growth is inhibited by tubulin inhibitor 6 at an IC50 of 840 nM.
Physicochemical Properties
| Molecular Formula | C20H14NO2SCL |
| Molecular Weight | 367.84866 |
| Exact Mass | 367.04 |
| Elemental Analysis | C, 65.30; H, 3.84; Cl, 9.64; N, 3.81; O, 8.70; S, 8.72 |
| CAS # | 105925-39-1 |
| Related CAS # | 105925-39-1 |
| PubChem CID | 2359994 |
| Appearance | White to off-white solid powder |
| LogP | 5.2 |
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 3 |
| Rotatable Bond Count | 2 |
| Heavy Atom Count | 25 |
| Complexity | 482 |
| Defined Atom Stereocenter Count | 0 |
| InChi Key | CSWHYHPUEDNIQY-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C20H14ClNO2S/c1-24-15-9-6-13(7-10-15)20(23)22-16-4-2-3-5-18(16)25-19-11-8-14(21)12-17(19)22/h2-12H,1H3 |
| Chemical Name | (2-chlorophenothiazin-10-yl)-(4-methoxyphenyl)methanone |
| Synonyms | Tubulin inhibitor 6; iHAP1; CUN25391; CUN 25391; CUN-25391 |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| Targets | tubulin polymerization (IC50 = 0.87 μM) |
| ln Vitro | Tubulin inhibitor 6 exhibits antiproliferative activity at submicromolar levels[1]. |
| References |
[1]. N-benzoylated phenoxazines and phenothiazines: synthesis, antiproliferative activity, and inhibition of tubulin polymerization. J Med Chem. 2011 Jun 23;54(12):4247-63. |
Solubility Data
| Solubility (In Vitro) |
DMSO: 20~62.5 mg/mL (54.4~169.9 mM) Ethanol: ~5 mg/mL (~13.6 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 6.25 mg/mL (16.99 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 900 μL corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7185 mL | 13.5925 mL | 27.1850 mL | |
| 5 mM | 0.5437 mL | 2.7185 mL | 5.4370 mL | |
| 10 mM | 0.2718 mL | 1.3592 mL | 2.7185 mL |