Tsugaric acid A can significantly inhibit the formation of superoxide anion. Tsugaric acid A also protects human keratinocytes from ultraviolet B (UVB)-induced damage. Tsugaric acid A protects keratinocytes from photodamage.

Physicochemical Properties

Molecular Formula	C32H50O4
Molecular Weight	498.7370
Exact Mass	498.37
CAS #	174391-64-1
PubChem CID	71207558
Appearance	White to off-white solid powder
Density	1.1±0.1 g/cm3
Boiling Point	577.3±50.0 °C at 760 mmHg
Melting Point	181 - 182 °C
Flash Point	174.4±23.6 °C
Vapour Pressure	0.0±3.4 mmHg at 25°C
Index of Refraction	1.536
LogP	9.94
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	7
Heavy Atom Count	36
Complexity	969
Defined Atom Stereocenter Count	0
InChi Key	FIWGZIBLJWZUEA-UHFFFAOYSA-N
InChi Code	InChI=1S/C32H50O4/c1-20(2)10-9-11-22(28(34)35)23-14-18-32(8)25-12-13-26-29(4,5)27(36-21(3)33)16-17-30(26,6)24(25)15-19-31(23,32)7/h10,22-23,26-27H,9,11-19H2,1-8H3,(H,34,35)
Chemical Name	2-(3-acetyloxy-4,4,10,13,14-pentamethyl-2,3,5,6,7,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-17-yl)-6-methylhept-5-enoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Additional Infomation

Tsugaric acid A has been reported in Ganoderma and Ganoderma lucidum with data available.

Solubility Data

Solubility (In Vitro)

DMSO : ~10 mg/mL (~20.05 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (5.01 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.01 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0051 mL	10.0253 mL	20.0505 mL
	5 mM	0.4010 mL	2.0051 mL	4.0101 mL
	10 mM	0.2005 mL	1.0025 mL	2.0051 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.