Topoisomerase I inhibitor 21 (Compound 3e) inhibits Topoisomerase I by stabilizing the enzyme-DNA complex. Topoisomerase I inhibitor 21 exhibits antiproliferative activity in 39 human cancer cells (JFCR39) with an average GI50 of 39 nM.

Physicochemical Properties

Molecular Formula	C27H27N3O4
Molecular Weight	457.52
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Topoisomerase I
ln Vitro	Topoisomerase I inhibitor 21 inhibits the proliferation of cancer cells MCF7, U251, HCT-166, A549, LOX-IMVI, SK-OV-3, ACHN, MKN28 and DU145 with EC50 <50 nM[1].
References	[1]. Design, synthesis, and biological evaluation of novel benzo6,7indolo3,4-cisoquinolines as anticancer agents with topoisomerase I inhibition. Bioorg Med Chem Lett. 2024 Mar 20:129710.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1857 mL	10.9285 mL	21.8570 mL
	5 mM	0.4371 mL	2.1857 mL	4.3714 mL
	10 mM	0.2186 mL	1.0928 mL	2.1857 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.