Physicochemical Properties
| CAS # | 2770804-58-3 |
| Appearance | Typically exists as solid at room temperature |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| Targets | Topoisomerase I Topoisomerase II |
| ln Vitro | Compound 1a, or topoisomerase I/II inhibitor 2, shows stronger inhibitory efficacy on two human hepatocellular carcinoma cell lines (HuH7, LM9) and excellent anti-proliferative activity in cancer cell lines (0-150 μM; 72 hours)[1]. HuH7 cells show no harm from topoisomerase I/II inhibitor 2 (20 μM; 24 hours); LM9 cells show moderate damage[1]. Huh7 and LM9 cell proliferation is inhibited by topoisomerase I/II inhibitor 2 (1.25-8 μM; 1-2 weeks) in a concentration-dependent manner[1]. In LM9 and HuH7 cells, topoisomerase I/II inhibitor 2 (1.25-8 μM; 24 hours) exhibits a good concentration-dependent inhibitory effect on migration and invasion[1]. In LM9 and HuH7 cells, topoisomerase I/II inhibitor 2 (0–20 μM; 24 hours) can suppress the production of matrix metalloproteinases-9 (MMP-9)[1]. Topoisomerase I/II inhibitor 2 (0–20 μM; 48 hours) stops the cell cycle at the G2/M phase, which prevents cells from proliferating[1]. In a concentration-dependent manner, topoisomerase I/II inhibitor 2 (3.5-20 μM; 48 hours) can damage mitochondrial function and cause cell apoptosis[1]. |
| Cell Assay |
Cell Proliferation Assay Cell Types: LM9, HuH7, SK-hep-1, HepG2, HT-29, HCT-116, RKO, SW480, MCF-7, MDA-B-231, HGC-27, SGC-7901, BGC-823, A549, U251, HL-60, LO2[1] Tested Tested Concentrations: 0-150 μM Incubation Duration: 72 hrs (hours) Experimental Results: Displayed favorable anti-proliferative activity and had better inhibitory activity on two human hepatocellular carcinoma cell lines (HuH7, LM9). Western Blot Analysis Cell Types: LM9 and HuH7 cells[1] Tested Tested Concentrations: 0, 3.75, 7.5, 15 μM in LM9; 0, 5, 10, 20 μM in HuH7 Incubation Duration: 48 hrs (hours) Experimental Results: Inhibited the expression of MMP-9. Cell Cycle Analysis Cell Types: LM9 and HuH7 cells[1] Tested Tested Concentrations: 0, 3.75, 7.5, 15 μM in LM9; 0, 5, 10, 20 μM in HuH7 Incubation Duration: 48 hrs (hours) Experimental Results: Inhibited cells proliferation by blocking cell cycle at the G2/M phase. Apoptosis Analysis Cell Types: LM9 and HuH7 cells[1] Tested Tested Concentrations: 3.5, 7, 14 μM in LM9; 5, 10, 20 μM in HuH7 Incubation Duration: 48 hrs (hours) Experimental Results: Induced apoptosis in a dose-dependent manner. |
| References | [1]. Deng X, et al. Design, synthesis and anti-hepatocellular carcinoma activity of 3-arylisoquinoline alkaloids. Eur J Med Chem. 2022;228:113985. |
Solubility Data
| Solubility (In Vitro) | May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |