Topo I/II-IN-1 (compound 7t) is a potent dual inhibitor of Topo I and Topo II. Topo I/II-IN-1 exhibits significant cytotoxicity against MCF-7 breast cancer cell line with an IC50 value of 7.45 μM.

Physicochemical Properties

Molecular Formula	C15H13N3S2
Molecular Weight	299.41
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Design, synthesis and anticancer evaluation of 4-Substituted 5,6,7,8-tetrahydrobenzo4,5thieno2,3-dpyrimidines as dual topoisomerase I and II inhibitors. Bioorg Chem. 2025 Jan;154:108043.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.3399 mL	16.6995 mL	33.3990 mL
	5 mM	0.6680 mL	3.3399 mL	6.6798 mL
	10 mM	0.3340 mL	1.6700 mL	3.3399 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.