Physicochemical Properties
| Molecular Formula | C₁₄H₁₃N₃O₂S |
| Molecular Weight | 287.34 |
| Exact Mass | 287.073 |
| CAS # | 78338-22-4 |
| PubChem CID | 2724414 |
| Appearance | Green to dark green solid powder |
| Density | 0.82 g/mL at 25 °C(lit.) |
| Boiling Point | 116-117 °C(lit.) |
| Flash Point | 46 °F |
| Index of Refraction | n20/D 1.389(lit.) |
| LogP | 2.813 |
| Hydrogen Bond Donor Count | 2 |
| Hydrogen Bond Acceptor Count | 5 |
| Rotatable Bond Count | 0 |
| Heavy Atom Count | 20 |
| Complexity | 414 |
| Defined Atom Stereocenter Count | 0 |
| InChi Key | JOIRQYHDJINFGA-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C12H9N3S.C2H4O2/c13-7-1-3-9-11(5-7)16-12-6-8(14)2-4-10(12)15-9;1-2(3)4/h1-6,13H,14H2;1H3,(H,3,4) |
| Chemical Name | (7-aminophenothiazin-3-ylidene)azanium;acetate |
| Synonyms | Thionine acetate3,7-Diamino-5-phenothiazinium acetate Lauth's violet |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| References | The use of filter paper discs impregnated with thionin acetate, basic fuchsin and thionin blue in the identification of Brucella species[J]. The Onderstepoort journal of veterinary research, 1990, 57(3):197-199 |
| Additional Infomation | Thionine acetate is an organic salt. It has a role as a fluorochrome. It contains a thionine cation. |
Solubility Data
| Solubility (In Vitro) | DMSO : ~41.67 mg/mL (~145.02 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.24 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.24 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4802 mL | 17.4010 mL | 34.8020 mL | |
| 5 mM | 0.6960 mL | 3.4802 mL | 6.9604 mL | |
| 10 mM | 0.3480 mL | 1.7401 mL | 3.4802 mL |