Tetramethylthiuram monosulfide (TMTM) is a bioactive compound. Tetramethylthiuram monosulfide (TMTM) may be used in rubber and a variety of biochemical studies.

Physicochemical Properties

Molecular Formula	C6H12N2S3
Molecular Weight	208.37
Exact Mass	208.016
CAS #	97-74-5
PubChem CID	7347
Appearance	Yellow powder
Density	1.3±0.1 g/cm3
Boiling Point	260.9±23.0 °C at 760 mmHg
Melting Point	107-111 °C(lit.)
Flash Point	111.6±22.6 °C
Vapour Pressure	0.0±0.5 mmHg at 25°C
Index of Refraction	1.641
LogP	0.87
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	2
Heavy Atom Count	11
Complexity	147
Defined Atom Stereocenter Count	0
SMILES	S=C(N(C)C)SC(N(C)C)=S
InChi Key	REQPQFUJGGOFQL-UHFFFAOYSA-N
InChi Code	InChI=1S/C6H12N2S3/c1-7(2)5(9)11-6(10)8(3)4/h1-4H3
Chemical Name	dimethylcarbamothioyl N,N-dimethylcarbamodithioate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Toxicity/Toxicokinetics	Interactions In adult female wistar rats, pretreated by gavage with 16 or 256 uM/kg cyanamide, tetramethylthiuram disulfide (TMTD), tetramethylthiuram monosulfide (TMTM), ziram or zineb 90 min or 18 hr before admin of 2 g ethanol/kg ip, the blood acetaldehyde levels were significantly incr for 90-240 min after ethanol admin (exceptions were noted after exposure to zineb for 90 min or to low dosed cyanamide for 18 hr). After pretreatment for identical periods with ANTU or ANIT at doses extending into the LD50 range, the blood acetaldehyde levels of rats given the same dose of ethanol remained uninfluenced. The incr in blood acetaldehyde recorded after 16 uM/kg TMTM and TMTD orally remained detectable for up to 48 hr. Onset of the cyanamide action /resulted/ after 45 min. ...
References	[1]. Coleman, et al. Vibrational spectra of thiuram sulfides. Normal coordinate analysis of tetramethylthiuram mono-, di-, and tetrasulfides. Journal of Polymer Science, Polymer Physics Edition.Volume: 12.Issue: 5.Pages: 1001-13.Journal.1974. [2]. Yingtao Sun, et al. Synergistic Effect of Copper Sulfate and Tetramethylthiuram Monosulfide Induced Metal-Coordination Cross-Linking in Nitrile–Butadiene Rubber. 25 April 2021.
Additional Infomation	Tetramethylthiuram monosulfide is a Standardized Chemical Allergen. The physiologic effect of tetramethylthiuram monosulfide is by means of Increased Histamine Release, and Cell-mediated Immunity.

Solubility Data

Solubility (In Vitro)

DMSO: 250 mg/mL (1199.79 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (9.98 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (9.98 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.7992 mL	23.9958 mL	47.9916 mL
	5 mM	0.9598 mL	4.7992 mL	9.5983 mL
	10 mM	0.4799 mL	2.3996 mL	4.7992 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.