Telomerase-IN-2 is a novel inhibitor of telomerase that inhibits the activity of dyskeratin by reducing its expression, with an IC50 value of 0.89 µM. Telomerase-IN-2 has anticancer effects.

Physicochemical Properties

Molecular Formula	C34H39N3O9
Molecular Weight	633.688169717789
Exact Mass	633.27
CAS #	1610878-54-0
PubChem CID	76285870
Appearance	White to off-white solid powder
LogP	3.8
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	11
Rotatable Bond Count	13
Heavy Atom Count	46
Complexity	1020
Defined Atom Stereocenter Count	0
SMILES	O(C1C(C2C(=CC(=CC=2OC=1C1C=C(C(=C(C=1)OC)OC)OC)OC)OC)=O)CCCCN1CCN(C(C2C=CN=CC=2)=O)CC1
InChi Key	HDOUAPSDPPFIGT-UHFFFAOYSA-N
InChi Code	InChI=1S/C34H39N3O9/c1-40-24-20-25(41-2)29-26(21-24)46-31(23-18-27(42-3)32(44-5)28(19-23)43-4)33(30(29)38)45-17-7-6-12-36-13-15-37(16-14-36)34(39)22-8-10-35-11-9-22/h8-11,18-21H,6-7,12-17H2,1-5H3
Chemical Name	5,7-dimethoxy-3-[4-[4-(pyridine-4-carbonyl)piperazin-1-yl]butoxy]-2-(3,4,5-trimethoxyphenyl)chromen-4-one
Synonyms	Telomerase-IN-2
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Telomerase ( IC50 = 0.89 µM )
References	[1]. Discovery of new chromen-4-one derivatives as telomerase inhibitors through regulating expression of dyskerin. J Enzyme Inhib Med Chem. 2018 Dec;33(1):1199-1211.

Solubility Data

Solubility (In Vitro)

DMSO : ~250 mg/mL (~394.5 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (3.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (3.28 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.5781 mL	7.8903 mL	15.7806 mL
	5 mM	0.3156 mL	1.5781 mL	3.1561 mL
	10 mM	0.1578 mL	0.7890 mL	1.5781 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.