Tau tracer 2 (Pl-2620) is a Tau tracer used for imaging Tau protein aggregates. Tau tracer 2 may be utilized to diagnose neurodegenerative diseases.

Physicochemical Properties

Molecular Formula	C15H9FN4
Molecular Weight	264.2572
Exact Mass	264.081
CAS #	2173361-80-1
Related CAS #	2173361-80-1 (F19);2173353-61-0 (F18);
PubChem CID	134202705
Appearance	Off-white to light yellow solid powder
LogP	2.6
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Heavy Atom Count	20
Complexity	353
Defined Atom Stereocenter Count	0
SMILES	FC1C([H])=C(C([H])=C([H])N=1)C1C([H])=C([H])C2=C(N=1)N([H])C1C([H])=C([H])N=C([H])C2=1
InChi Key	JUPWIBZGGFVBCU-UHFFFAOYSA-N
InChi Code	InChI=1S/C15H9FN4/c16-14-7-9(3-6-18-14)12-2-1-10-11-8-17-5-4-13(11)20-15(10)19-12/h1-8H,(H,19,20)
Chemical Name	11-(2-fluoropyridin-4-yl)-4,8,10-triazatricyclo[7.4.0.02,7]trideca-1(9),2(7),3,5,10,12-hexaene
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Using Positron Emission Tomography (PET) imaging, Tau tracer 2 (compound F-3a) can be used to selectively detect Tau aggregate-associated illnesses and abnormalities, such as Alzheimer's disease and other tauopathies[1].
References	[1]. Compounds for imaging tau protein aggregates. WO2018015549A1.

Solubility Data

Solubility (In Vitro)

DMSO : 16.67 mg/mL (63.08 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.67 mg/mL (6.32 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.67 mg/mL (6.32 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1.67 mg/mL (6.32 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.7842 mL	18.9208 mL	37.8415 mL
	5 mM	0.7568 mL	3.7842 mL	7.5683 mL
	10 mM	0.3784 mL	1.8921 mL	3.7842 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.