TRIA-662 (1-Methylnicotinamide chloride) is an endogenously produced metabolite with antithrombotic and anti~inflammatory activities.

Physicochemical Properties

Molecular Formula	C7H9CLN2O
Molecular Weight	172.61
Exact Mass	172.04
CAS #	1005-24-9
Related CAS #	TRIA-662-d3;1218993-18-0
PubChem CID	70495
Appearance	White to off-white solid powder
Melting Point	207-209℃ (water acetone )
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	1
Heavy Atom Count	11
Complexity	136
Defined Atom Stereocenter Count	0
SMILES	[Cl-].O=C(C1C([H])=C([H])C([H])=[N+](C([H])([H])[H])C=1[H])N([H])[H]
InChi Key	BWVDQVQUNNBTLK-UHFFFAOYSA-N
InChi Code	InChI=1S/C7H8N2O.ClH/c1-9-4-2-3-6(5-9)7(8)10;/h2-5H,1H3,(H-,8,10);1H
Chemical Name	1-methylpyridin-1-ium-3-carboxamide;chloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Human Endogenous Metabolite
ln Vitro	G-actin polymerization is observed after 12 and 24 hours of treatment with TRIA-662 (0.1–10 μM) [1]. Endothelial cells demonstrate anti-inflammatory effect towards TRIA-662 (0.05-0.2 μM; 1-24 hours) [1].
Cell Assay	Immunofluorescence[1] Cell Types: HMECs Tested Concentrations: 0.1 and 10 μM Incubation Duration: 12 and 24 hrs (hours) Experimental Results: demonstrated polymerization of the G-actin, and strong, clearly visible, parallel F-actin fibers at 10 μM.
References	[1]. Nanomechanical sensing of the endothelial cell response to anti-inflammatory action of 1-methylnicotinamide chloride. Int J Nanomedicine. 2013;8:2757-67.

Solubility Data

Solubility (In Vitro)

H2O: 110 mg/mL (637.27 mM) DMSO: 2 mg/mL (11.59 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (7.24 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (7.24 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 3: 100 mg/mL (579.34 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.7934 mL	28.9670 mL	57.9341 mL
	5 mM	1.1587 mL	5.7934 mL	11.5868 mL
	10 mM	0.5793 mL	2.8967 mL	5.7934 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.