TMN355 is a potent chemical cyclophilin A inhibitor that reduces foam cell formation and cytokine secretion. TMN355 may be used in atherosclerosis.

Physicochemical Properties

Molecular Formula	C21H14CLFN2O2
Molecular Weight	380.8034
Exact Mass	380.073
CAS #	1186372-20-2
PubChem CID	44249042
Appearance	White to yellow solid powder
LogP	5.47
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	2
Heavy Atom Count	27
Complexity	552
Defined Atom Stereocenter Count	0
InChi Key	SFNLLCUAISZNRV-UHFFFAOYSA-N
InChi Code	InChI=1S/C21H14ClFN2O2/c22-16-10-5-11-17(23)18(16)20(26)25-21(27)24-19-14-8-3-1-6-12(14)13-7-2-4-9-15(13)19/h1-11,19H,(H2,24,25,26,27)
Chemical Name	2-chloro-N-[(9H-fluoren-9-ylamino)carbonyl]-6-fluoro-benzamide
Synonyms	Compound 3i TMN 355 TMN-355 TMN355
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Cyclophilin A protein expression was reduced by 75.9% when TMN 355 (0.5–10 μM) was administered for three to nine hours. After six hours of activation, 1 μM TMN 355 inhibits cyclophilin A [1].
Cell Assay	Western Blot Analysis[1] Cell Types: THP Cell Line Tested Concentrations: 0.5, 1, 2.5, 5 and 10 μM Incubation Duration: 3, 6 and 9 hrs (hours) Experimental Results: Resulted in 75.9% reduction in cyclophilin A protein expression.
References	[1]. Cyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus. Cardiovasc Diabetol. 2016 Nov 3;15(1):152.

Solubility Data

Solubility (In Vitro)

DMSO : ~31.25 mg/mL (~82.06 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (5.46 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6261 mL	13.1303 mL	26.2605 mL
	5 mM	0.5252 mL	2.6261 mL	5.2521 mL
	10 mM	0.2626 mL	1.3130 mL	2.6261 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.